Author Archives: Allison Land

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November 23, 2021 Aidan Forberg, Lauren Buysse, Eli Landas Introduction: After a few trials with the restriction digest we have been able to purify our product and insert it into the pcDNA vector via a ligation and transformation. We were … Continue reading

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Finalizing Our Work with miRNA 1207-3P and miRNA 4297

November 9th, 2021 D’Aaliyah Johnson, Matthew Trenne, Carter Beck Introduction: Last Semester, in the spring of 2021 we chose two miRNAs to work with and investigate their effect on APOBEC3A and APOBEC3B, these miRNAs were hsa-mir-4297 and hsa-mir-1207-3p. In our … Continue reading

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Return of miRNA-4314 & miRNA-4786-3P for Fall of 2021

October 19, 2021 Aidan Forberg, Lauren Buysse, Eli Landas Introduction: Last semester, we successfully inserted our miRNA hsa-mir-4314 into the pMiniT plasmid. For our other miRNA, hsa-mir-4786-3P, we did not receive positive sequencing back, which has us behind where we … Continue reading

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miRNA 1207-3P and miRNA 4297 Returns for Fall 2021

October 5, 2021 D’Aaliyah Johnson, Matthew Trenne, Carter Beck Introduction: During spring of 2021 our lab group chose two microRNAs to observe how they interact with APOCEC3A and APOBEC3B. Our first microRNA is hsa-mir-4297, which has a target score of … Continue reading

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Colony PCR

April 17, 2021 Catie Fleischer and Carter Beck Introduction: We are part of the Cancer an Immunity Stream with Dr. Land at Minnesota State University, Mankato. Our research is focused on how APOBEC3A and APOBEC3B interact with miRNAs (replicated fragments … Continue reading

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