Author Archives: Yongtao Zhu

Finalization of the DNA cloning

April 9th, 2022 James Hawco and Jake Lohn Introduction: Since our last blog post, we have conducted DNA ligation, transformation, colony PCR, and confirmation for the upstream region of our target gene. After confirmation of our upstream region, we have … Continue reading

Posted in Bacteria & Disease | Leave a comment

DNA Digestion and Ligation

1 Mar 2022 Losel Baro, Nikita Damyan, and Zhelah Guzeh Introduction: We were selected for the Bacteria and Disease research stream at Minnesota State University Mankato, led by Dr. Yongtao Zhu. The purpose of our stream is to delete the … Continue reading

Posted in Bacteria & Disease | Leave a comment

Getting Started with PCR

February 11th, 2022 James Hawco and Jake Lohn Introduction: The Bacteria and Disease research stream at Minnesota State University, Mankato, attempts to create a vaccine for bacterial cold-water disease (BCWD) in salmonoid fishes. This process is being done with the … Continue reading

Posted in Bacteria & Disease | Leave a comment

Our Continuation of Research with Flavobacterium psychrophilum

November 12, 2021 Emily Schmidtbauer and Kaela Wierenga Introduction: Since September 24, 2021, we have worked to insert our upstream region of the endonuclease-encoding gene into our construct pSW01-3. We were able to successfully insert our upstream region creating our … Continue reading

Posted in Bacteria & Disease | Leave a comment

Continued Work on SprB Motility Adhesin Gene in Flavobacterium psychrophilum

October 29, 2021 Marley Fuhrman Last semester my lab partner Abby Mueller and I successfully created a plasmid with the downstream insert of our SprB gene and named it pFM01. This semester I’m continuing research by inserting the upstream gene … Continue reading

Posted in Bacteria & Disease | Leave a comment