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RISEbio is a National Science Foundation funded scholarship program at Minnesota State University, Mankato. This program gives first year students interested in pursuing a degree in biology the opportunity to engage in real-world research with faculty and graduate students.
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Category Archives: Bacteria & Disease
SprB Mutant Confirmation and Phenotype Test
By: Nikita Damyan, Losel Baro, Zhelah Guzeh Introduction: We have come to the end of our group’s part of the research and process to create a deletion mutant of the adhesin and motility gene, sprB, in the fish pathogen Flavobacterium psychrophilum. … Continue reading
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Finalization of the reinsertion of the fpsY gene into Flavobacterium psychrophilum
James Hawco and Jake Lohn Introduction: The majority of our time from the last post to this one has been waiting for various colonies to grow since conjugation is a process that takes place on different media for it to … Continue reading
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Deletion of a gliding motility-related gene
Zhelah Guzeh. Losel Baro, and Nikita Damyan Introduction We were selected for the Bacteria and Disease research stream at Minnesota State University Mankato, led by Dr. Yongtao Zhu. The purpose of our stream is to delete the sprB gene … Continue reading
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Preparation for the reinsertion of the fpsY gene into Flavobacterium psychrophilum
October 4, 2022 James Hawco and Jake Lohn Introduction: Since our last blog post, we have successfully obtained the fpsY deletion mutant from a wildtype background of F. psychrophilum. The mutant has shown a few differences (phenotypes) compared to the … Continue reading
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Colony PCR
Losel Baro, Nikita Damyan, Zhelah Guzeh Introduction: Over the last couple of weeks, we have finished working with the cloning of upstream region of the sprB gene and have begun work on the downstream region. The work we have been … Continue reading
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